Establishment of Quantitative SPR Assay for Antibodies Against Human Platelet Antigen-1a / 中国实验血液学杂志

OBJECTIVE@#To establish quantitative surface plasmon resonance (SPR) assay for antibodies against human platelet antigen-1a (HPA-1a).@*METHODS@#Recombinant protein was fixed on the chip surface by amino coupling method. SPR assay was used to detect the standard antibodies against HPA-1a at different conceatration. The optimal experimental parameters were determined, and standard curves were constructed with linear regression. Moreover, the sensitivity, specificity, accuracy and precision of the assay were evaluated.@*RESULTS@#The quantitative SPR assay for HPA-1a antibodies was established. The determination ranges were 0-20 IU, with accuracy (recovery rate) was 97.75%-103.08%. The intra-assay precision [coefficients of variation (CV)] was 3.53%-4.29%, and the inter-assay precision (CV) was 2.08%-4.40%. For specificity test, several kinds of monoclonal and human antibodies against platelet membrane protein were tested and no positive result was observed.@*CONCLUSION@#The established quantitative SPR assay for HPA-1a antibodies shows good sensitivity, specificity, accuracy and precision, and this rapid and simple method provides a new reference method for scientific research and clinical antibody detection.

Expression and significance of monoacylglycerol Oacyltransferase 2 (MOGAT2) in pterygium / 眼科新进展

Objective To investigate the expression and significance of monoacylglycerol O-acyltransferase 2 (MOGAT2) in the tissues of pterygium.Methods Immunohistochemistry staining methods were adopted to detect the expression of MOGAT2 in 54 patients of pterygia (primary in 50 patients and recurrence in 4 patients)and 18 patients of normal conjunctival tissues.The semi-quantitative integration method was used to analyze the strength of immunohistochemical expression,and the results were compared and statistically analyzed,and the relationship between the expression of MOGAT2 in the pterygium group and the factors such as age,sex,and clinical pathological stage was analyzed.Results Immunohistochemical staining showed that,in 54 patients of pterygium,MOGAT2 was strongly expressed in 8 patients,positive expression in 29,weakly positive expression in 8 and negative expression in 9,while in 18 normal conjunctival tissues,strongly positive expression of MOGAT2 was found 1 patient,positive expression in 6,weakly positive expression in 3,negative expression in 8,respectively.Rank-sum test showed that the difference in MOGAT2 expression in the pterygium and normal conjunctiva tissues was statistically significant(Z =-2.403,P =0.016).There was no significant difference in the expression of MOGAT2 between the different age and the sex patients with pterygium (all P ＞ 0.05),and there was no significant correlation of MOGAT2 expression with education,occupation,income,protection in outdoor work,smoking,drinking,blood sugar,blood pressure,primary and recurrence pterygium,but significant correlation with the clinical stage of pterygium,that is,MOGAT2 expression in advanced pterygium was overexpressed when compared with tumor tissues in quiescent stage (P ＜ 0.05).Conclusion MOGAT2 is highly expressed in pterygium tissues,and the expression in advanced pterygium was significantly upregulated,of which is related to the formation and development of pterygium.

Status que of acupuncture for strephenopodia after stroke / 中国针灸

In order to optimize acupuncture protocol for stephenipodia after stroke, related papers of the recent 20 years are retrieved. Interrelated factors which may influence the therapeutic effect of acupuncture are analyzed through the aspects such as acupoints, needling methods and opportunities of acupuncture. It is held that importance should be attached on relation between acupoints and anatomy during acupoint selection. Different acupuncture protocol can be adopted in different phases of stephenipodia according to its time of occurrence. And it is found that the combination of acupuncture and rehabilitation can reinforce the therapeutic effect. Relationship between effect of acupuncture and patient's condition, acupoint selection and prescription, acupuncture manipulations (including directions, angles, reinforcing and reducing, quantity of stimulus) are worth to be further studied. On the base of the above all mentioned studies, optimization can be carried out on selection of acupoints and acupuncture manipulations. And therapeutic effect can be enhanced when the optimized acupuncture protocol is combined with rehabilitation therapies.

Assessment of epidermal growth factor receptor status in glioblastomas

Our previous study showed that a newly designed tracer radioiodinated 6-[3-morpholinopropoxy]-7-ethoxy-4-[3'-iodophenoxy]quinazoline [[[125]I]PYK] is promising for the evaluation of the epidermal growth factor receptor [EGFR] status and prediction of gefitinib treatment of non-small cell lung cancer. EGFR is over-expressed and mutated also in glioblastoma. In the present study, the expressions and mutation of EGFR were tested with [[125]I] PYK in glioblastoma in vitro and in vivo to determine whether this could be used to predict the sensitivity of glioblastoma to gefitinib treatment. Glioblastoma cell lines with different expression of EGFR were tested. Growth inhibition of cell lines by gefitinib was assessed by the 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide [MTT] colorimetric assay. Uptake levels of [[125]I]PYK were evaluated in cell lines in vitro. Tumor targeting of [[125]I]PYK was examined by a biodistribution study and imaging by single photon emission computed tomography [SPECT]. High concentrations of gefitinib were needed to suppress EGFR-mediated proliferation. The uptake of [[125]I] PYK in cell lines in vitro was low, and showed no correlation with EGFR expression or mutation status. Biodistribution study and SPECT imaging with [[125]I]PYK for xenografts showed no [[125]I]PYK uptake. The results showed prediction of gefitinib effectiveness was difficult in glioblastoma by [[125]I]PYK, which might be due to the complicated expression of EGFR status in glioblastoma. Thus, new tracers for sites downstream of the mutant EGFR should be investigated in further studies

The role of acupuncture and moxibustion in organized medicine of pain / 中国针灸

The literature on acupuncture-moxibustion and acupuncture-moxibustion combined with other therapies for all kinds of pain as well as organized medicine of pain in recent ten years are analyzed. It is found out that application of single acupuncture-moxibustion has positive effect on most of pain, while acupuncture-moxibustion combined with other therapies could improve the efficacy in a certain extent. The organized medical model of pain, by integration of clinical paths and optimization of medical methods, could achieve best effect on pain. With acupuncture-moxibustion joining in the organized medical model of pain, the analgesia effect could be enhanced, medical cost decreased and patient's life quality improved. The suitable disease, clinical path, action function and mechanism need further evidence-based research.

Effects of benzene and selenium on telomerase in mouse lymphocytes in vivo / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study the effects of benzene and selenium on telomerase in mouse lymphocytes in vivo and evaluate telomerase activity as an early marker of benzene effects on lymphocytes.</p><p><b>METHODS</b>Male Kunming mice were divided randomly into 8 groups, including negative control group, reagent control group, 100 mg/kg benzene group, 200 mg/kg benzene group, 400 mg/kg benzene group, 200 mg/kg benzene + 0.75 mg/kg selenium group, 200 mg/kg benzene + 1.50 mg/kg selenium group and 200 mg/kg benzene + 3.00 mg/kg selenium group, 5 mice in each group. The mice in different groups were treated with different methods, once daily for 5 days. After 48 hours of the final exposure, lymphocytes were separated and the telomerase activities were detected with TRAPELISA.</p><p><b>RESULTS</b>Compared with negative and reagent control groups, the telomerase activity was increased after treatment with different dose of benzene and at the dose of 100 mg/kg benzene group it was significantly increased (P < 0.01). At the dose of 200 mg/kg benzene + 0.75 mg/kg selenium group, it was significantly increased (P < 0.01). Compared with the counterpart treated with 200 mg/kg benzene group, the expression of telomerase was increased at the different concentrations after treatment with benzene combined with selenium and it was significantly increased at the dose of 200 mg/kg benzene + 0.75 mg/kg selenium group (P < 0.05).</p><p><b>CONCLUSION</b>Increased telomerase activity in lymphocytes stimulated by benzene at different concentrations indicates activation and proliferation of these lymphocytes of mice in vivo. Telomerase activity is probably a sensitive early marker of lymphocyte proliferation by benzene. Selenium can upregulate the telomerase activity.</p>